primer5 function introduction
1. The main functions of the software are divided into four types, namely primer design, restriction endonuclease site analysis, and DNA analysis. Motif search and homology analysis functions. The first three are its main functions. In addition, the software has some special functions, the most important of which is the design of degenerate primers, as well as sequence "reading", DNA Interchange with protein sequences, voice prompt keyboard input, etc.
2. You can also convert DNA according to the corresponding genetic code rules according to the source of template DNA. and amino acid sequence. The software provides a total of eight different genetic code rules for biological substructures for users to choose, including Ciliate Macron (Ciliate Macronuclear), invertebrate mitochondria (Invertebrate Mitochondrion), Mycoplasma (Mycoplasma), plant mitochondria (Plant Mitochondrion), protozoan mitochondria (Protozoan Mitochondrion), general standard (Standard), vertebrate mitochondria (Vertebrate Mito-chondrion) and yeast mitochondria (Yeast Mitochondrion).
primer5 features description
It is a software for designing primers. The software is divided into four parts. The most commonly used ones are primer design, restriction endonuclease site analysis and DNA motif search.
We can directly submit the template sequence to a specific web page, such as the famous primer3, to get the designed primers, or we can run professional primer design software on the local computer.
It brings great convenience to professionals. In addition, the latest version brought to you by the editor is more powerful and based on the basic principles of primer design, but it is faster and more automated.
primer5 usage tutorial
1. Unzip the downloaded primer primer5.0 to the specified location, double-click "Primer Premier 5.exe" to run and enter the software interface;
2. Click Primer in the pop-up interface;
3. Click Search in the interface. After setting up, click OK;
4. Click Align in the pop-up interface to perform quick conversion, and click OK to convert;
primer5 update log
1. The details are more outstanding!
2.BUG gone without a trace
Huajun editor recommends:
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