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dnastar software features
Makes it easy for evolutionary biologists to perform multiple and pairwise sequence alignments, identify genes, assemble overlapping fragments of Sanger sequences, create virtual clones and design primers, taking advantage of a variety of comprehensive analysis tools
Includes all the tools needed for next-generation sequence assembly and analysis in a single integrated software package.
It is a software suite designed based on Protean 3D and NovaFold. The former is an application for exploring the structure, motion and function of macromolecules, and the latter predicts the three-dimensional structure of protein sequences.
Using the Lasergene Cloning Suite, you can edit and annotate sequences, create detailed circular or linear plots, and implement automated virtual cloning using a variety of cloning methods, including Gateway Multisite, TA, TOPO, and restricted cloning.
DNAstar function introduction
Comprehensive biomedical software for DNA and protein sequence analysis, contig assembly and genetic engineering management. Contains 7 modules:
SeqBuilder --Visualization and sequence editing
SeqMan Pro -- Sequence assembly and SNP discovery
MegAlign --sequence combination
PrimerSelect --oligo primer design
Protean – Protein structure analysis and prediction
GeneQuest-Gene search
EditSeq--Tool for importing special files
dnastar installation steps
1. Download the dnastar compressed package from this site and decompress it. Find "Lasergene710WinInstall.exe" in the decompressed directory and double-click to run the installation;
2. After entering the software installation wizard, click “Next”;
3. After reading the user agreement, click "YES" to agree to the agreement;
4. After selecting the installation directory of the file, click "Next". If you want to customize the installation directory, click "Browse..." to select the installation location.
5. Then the software installation information is displayed, and the user can directly click "Next";
6. The software is being installed, please wait until the installation is completed;
How to use dnastar
Use DNAStar to open gene sequences
1. Copy sequence
After we search for the sequence, click to open the sequence. We can see that the first half is a detailed description of the sequence, followed by the original text of the sequence. We select the sequence area, right-click and "Copy".
2. Paste directly
After copying the sequence, open DNAStar - "EditSeq", right-click the blank space of the newly created file, select "Paste", a prompt will appear, click OK, and the sequence will be copied. Remember to click "Save" after the analysis is completed, otherwise you will have to download it again.
3. Create a new txt file
In fact, "EditSeq" in DNAStar can also recognize txt files. After pasting the copied sequence into the txt file and then dragging it to EditSeq, the sequence file can also be opened, but we need to remove the numbers in front of the sequence to keep the sequence continuous. Just sex.
dnastar instructions for use
Add new enzymes
You can manually add new enzymes to SeqBuilder using the Enzyme Manager.
1.Select ENZYMES>New Enzyme. Enzyme Manager appears.
2. Enter the name of the new restriction enzyme in Enzyme name.
3. Type the recognition sequence in the recognition sequence text box.
4. Select the class of enzyme (Exact, Random or Unknown) from the Class list box.
If the course is precise, you can set the upper line and lower cutting point. Drag the arrows above and below the recognition sequence to the appropriate locations. The top arrow indicates the upper cutting site and the lower arrow indicates the lower cutting site.
5. (Optional) Enter the name of any commercially available isomers in the "Isoschizomers" list box by clicking in the box and typing isoschizomers.
6. (Optional) Enter the supplier's three-letter code into the Suppliers list box by clicking in the box to enter the three-letter code.
7. (Optional) Enter the cost value and unit number, and enter the unit cost.
8. (Optional) Define enzyme availability by checking On Hand or Procurable.
9. Click OK to add the new enzyme to the library.
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