Don’t you know how to use DNAMAN for primer design? If you don’t know how, you can come and learn the DNAMAN primer design method with the editor. I hope that through the study of this tutorial, it can help everyone better understand the DNAMAN software.
1. First, you must obtain the target gene for which you want to design primers.
2. Then, open the DNAMAN software, click the Primer option on the software menu bar after opening the software, and select the Load Primer---From Input option in the pop-up selection.
3. After importing the file, click the Primer option on the menu bar and select Melting Temperature from the pop-up options.
4. Open the Melting Temperature dialog box. You can modify the Thermo option. The Thermo value is generally between 55°C and 70°C.
5. From the picture above, you can see that the Tm value of our 20 bases is only 49 degrees, which is obviously too low and the number of bases needs to be increased. We take the first 24 bases and paste them in. Click Show Tm below to see the Tm value of this primer. We find that it is 60.3 degrees, which is very suitable. In this way, the forward primer is designed and the 24 base sequences are saved.
The above is the DNAMAN primer design method shared by the editor. Friends in need can take a look.
